Discovery | Light-sheet
openUC2 LightSheetBox
Build a Fully Automated Light Sheet Microscope
The openUC2 LightSheetBox enables the construction of a light sheet microscope with a minimal component count and compact footprint. Designed to work seamlessly with the CoreBox, ElectronicsBox, FluoBox, and InfinityBox, this kit provides everything needed for automated light sheet imaging in biological and volumetric applications.
Included Components
In Cubes
- Cylindrical Lens (100mm, adjustable for creating the light sheet)
- Sample Chamber (water-fillable with integrated NeoPixel LED for transmitted light illumination)
- Micrometer XYZ Stage (precise sample positioning with syringe sample holders)
Additional Accessories
- Solid Baseplate (10mm POM, 25×20 cm)
Features and Applications
- Compact Light Sheet Design: Based on the openSPIM configuration, this setup minimizes complexity while maintaining high performance. The entire assembly fits on a baseplate measuring only 25×20 cm.
- Automated Volume Imaging: Use the micrometer XYZ stage to move samples in all directions, enabling 3D imaging and volumetric scans.
- Light Sheet Generation: Collimate a single-mode, fiber-coupled 488nm laser and focus it into the back focal plane of a cylindrical lens. The astigmatism of the lens creates the light sheet, which illuminates the sample chamber.
- Orthogonal Fluorescence Detection: Perpendicular to the light sheet, the fluorescence imaging system uses an infinite-corrected objective, emission filter, tube lens, and monochrome camera to capture high-quality images.
- Software Integration: Controlled via the ImSwitch software and a streamlined UC2-eSP firmware, the system supports automated data acquisition and light sheet alignment.
Applications
This kit is ideal for imaging biological samples, such as living organisms, cells, or tissues, in a 3D environment. Its modular design ensures easy integration into existing setups, allowing advanced light sheet microscopy with minimal adjustments.
Explore Light Sheet Imaging Today
With the openUC2 LightSheetBox, bring advanced light sheet microscopy to your lab with an open-source, fully customizable approach. Expand your microscopy horizons with ease and precision.
Important: For the full light-sheet assembly, you need the following packages in addition to the Light-sheet box
